A blood sample is needed.

No special preparation is needed.

When the needle is inserted to draw blood, some people feel moderate pain. Others feel only a prick or stinging. Afterward, there may be some throbbing or a slight bruise. This soon goes away.

This test may be performed if:

• You have or are at risk for type 1 diabetes
• You appear to have an allergic response to insulin
• Insulin no longer seems to control your diabetes
• You are taking insulin to control your diabetes and your blood sugar level varies a lot, with both high and low numbers that can't be explained by the food you are eating relative to the timing of your insulin injections

Normally, there are no antibodies against insulin in your blood. Antibodies can be found in the blood of many people who are taking insulin to control diabetes.

Normal value ranges may vary slightly among different laboratories. Some labs use different measurements or test different samples. Talk to your health care provider about the meaning of your specific test results.

The presence of IgG and IgM antibodies against insulin can be part of the testing that diagnoses you with type 1 diabetes.

If you develop anti-insulin antibodies, insulin may not work as well, or it may not work at all. As a result, your blood sugar can be very high. Or the antibodies can also put you at risk for low blood sugar.

Your health care provider may suggest a different form of insulin to which your body may be less likely to form antibodies.

If the test shows a high level of IgE antibody against insulin, your body has developed an allergic response to the insulin.

• This could put you at risk for skin reactions where you inject insulin. You can also develop more severe reactions that affect your blood pressure or breathing.
• Medicines, such as antihistamines or low-dose injectable steroids, may help. Or you may need a treatment process called desensitization or a treatment to remove the antibodies from your blood.

There is little risk involved with having your blood taken. Veins and arteries vary in size from one person to another and from one side of the body to the other. Obtaining a blood sample from some people may be more difficult than from others.

Other risks of having blood drawn are slight, but may include:

• Excessive bleeding
• Fainting or feeling lightheaded
• Multiple punctures to locate veins
• Hematoma (blood building up under the skin)
• Infection (a slight risk any time the skin is broken)

Insulin antibodies - serum; Insulin Ab test; Insulin resistance - insulin antibodies; Diabetes - insulin antibodies

Atkinson MA, Mcgill DE, Dassau E, Laffel L. Type 1 diabetes mellitus. In: Melmed S, Auchus RJ, Goldfine AB, Koenig RJ, Rosen CJ, eds. Williams Textbook of Endocrinology. 14th ed. Philadelphia, PA: Elsevier; 2020:chap 36.

Chernecky CC, Berger BJ. Insulin and insulin antibodies - blood. In: Chernecky CC, Berger BJ, eds. Laboratory Tests and Diagnostic Procedures. 6th ed. Philadelphia, PA: Elsevier Saunders; 2013:682-684.

Kahn CR, Ferris HA, O'Neill BT. Pathophysiology of type 2 diabetes mellitus. In: Melmed S, Auchus RJ, Goldfine AB, Koenig RJ, Rosen CJ, eds. Williams Textbook of Endocrinology. 14th ed. Philadelphia, PA: Elsevier; 2020:chap 34.

Updated by: Robert Hurd, MD, Professor of Endocrinology and Health Care Ethics, Xavier University, Cincinnati, OH. Review provided by VeriMed Healthcare Network. Also reviewed by David Zieve, MD, MHA, Medical Director, Brenda Conaway, Editorial Director, and the A.D.A.M. Editorial team.