The health care provider removes a piece of tissue from your large intestine. This is done during a sigmoidoscopy or colonoscopy.

• The sample is sent to a lab.
• It is placed in a special dish that contains a gel. Bacteria and other organisms can grow in this gel. The dish is then stored at a certain temperature.
• The lab team checks the sample daily. They check to see if bacteria, viruses, or fungi have grown.

If certain germs grow, more tests will be done to identify them. This helps decide the best treatment.

There is no specific preparation needed for a culture itself. However, you will have to prepare for the sigmoidoscopy or colonoscopy if that is how your provider is getting the tissue sample. Speak with your provider about how to prepare.

Once the sample is taken, the culture does not involve you. Therefore, there is no pain.

Your provider may order this test if you have signs or symptoms of a large intestine infection. Colonic tissue culture is only done occasionally. More often, tests are done on stool to identify a large intestine infection. A colonic tissue culture is most often done only when other tests, such as a stool culture, could not identify the cause of infection.

A normal result means that no disease-causing organisms have grown in the lab dish.

Some "healthy" bacteria, called bowel flora, are normally found in the gut. The growth of such bacteria during this test does not mean there is an infection.

Normal value ranges may vary slightly among different laboratories. Talk to your provider about your test results.

An abnormal result means that disease-causing organisms have grown in the lab dish. These organisms may include:

• Clostridioides difficile bacteria
• Cytomegalovirus
• Mycobacterium tuberculosis bacteria
• Salmonella bacteria
• Shigella bacteria

These organisms may lead to colon infections or diarrhea.

There is very minimal risk associated with the procedure. In rare cases, excessive bleeding could occur when a tissue sample is taken. 

Colonic tissue culture

Fleckenstein JM. Approach to the patient with suspected enteric infection. In: Goldman L, Cooney KA, eds. Goldman-Cecil Medicine. 27th ed. Philadelphia, PA: Elsevier; 2024:chap 262.

Melia JMP, Sears CL. Infectious enteritis and proctocolitis. In: Feldman M, Friedman LS, Brandt LJ, eds. Sleisenger and Fordtran's Gastrointestinal and Liver Disease. 11th ed. Philadelphia, PA: Elsevier; 2021:chap 110.

Siddiqi HA, Rabinowitz S, Axiotis CA. Laboratory diagnosis of gastrointestinal and pancreatic disorders. In: McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 24th ed. Philadelphia, PA: Elsevier; 2022:chap 23.

Wojewoda CM, Stempak LM. Medical bacteriology. In: McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 24th ed. Philadelphia, PA: Elsevier; 2022:chap 57.

Updated by: Jenifer K. Lehrer, MD, Department of Gastroenterology, Aria - Jefferson Health Torresdale, Jefferson Digestive Diseases Network, Philadelphia, PA. Review provided by VeriMed Healthcare Network. Also reviewed by David C. Dugdale, MD, Medical Director, Brenda Conaway, Editorial Director, and the A.D.A.M. Editorial team.