Most of the time, the sample will be collected as a clean catch urine sample in your health care provider's office or your home. You will use a special kit to collect the urine.

A urine sample can also be taken by inserting a thin rubber tube (catheter) through the urethra into the bladder. This is done by someone in your provider's office or at the hospital. The urine drains into a sterile container, and the catheter is removed.

Rarely, your provider may collect a urine sample by inserting a needle through the skin of your lower abdomen into your bladder.

The urine is taken to a lab to determine which, if any, bacteria or yeast are present in the urine. This takes 24 to 48 hours.

If possible, collect the sample when urine has been in your bladder for 2 to 3 hours.

When the catheter is inserted, you may feel pressure. A special gel is used to numb the urethra.

Your provider may order this test if you have symptoms of a urinary tract infection or bladder infection, such as pain or burning when urinating.

You also may have a urine culture after you have been treated for an infection. This is to make sure that all of the bacteria are gone.

"Normal growth" is a normal result. This means that there is no infection.

Normal value ranges may vary slightly among different laboratories. Some labs use different measurements or test different samples. Talk to your provider about the meaning of your specific test results.

A "positive" or abnormal test is when bacteria or yeast are found in the culture. This likely means that you have a urinary tract infection or bladder infection.

Other tests may help your provider know which bacteria or yeast are causing the infection and which antibiotics will best treat it, if treatment is needed.

Sometimes more than one type of bacteria, or only a small amount, may be found in the culture.

There is a very rare risk for a hole (perforation) in the urethra or bladder if your provider uses a catheter.

You may have a false-negative urine culture if you have been taking antibiotics.

Culture and sensitivity - urine

Cooper KL, Badalato GM, Rutman MP. Infections of the urinary tract. In: Partin AW, Dmochowski RR, Kavoussi LR, Peters CA, eds. Campbell-Walsh-Wein Urology. 12th ed. Philadelphia, PA: Elsevier; 2021:chap 55.

Nicolle LE, Drekonja D. Approach to the patient with urinary tract infections. In: Goldman L, Cooney KA, eds. Goldman-Cecil Medicine. 27th ed. Philadelphia, PA: Elsevier; 2024:chap 263.

Updated by: Linda J. Vorvick, MD, Clinical Professor, Department of Family Medicine, UW Medicine, School of Medicine, University of Washington, Seattle, WA. Also reviewed by David C. Dugdale, MD, Medical Director, Brenda Conaway, Editorial Director, and the A.D.A.M. Editorial team.