Usually, you collect a small sample of stool at home. Sometimes, your health care provider may collect a small amount of stool from you during a rectal examination.

If the test is done at home, you will use a test kit. Follow the kit instructions exactly. This ensures accurate results. In brief:

• You collect a stool sample from 3 different bowel movements.
• For each bowel movement, you smear a small amount of the stool on a card provided in the kit.
• You mail the card to a laboratory for testing.

Be sure to keep the stool away from any urine. Mixing of urine and stool can spoil the sample.

Some foods can affect test results. Follow instructions about not eating certain foods before the test. These may include:

• Red meat
• Cantaloupe
• Uncooked broccoli
• Turnip
• Radish
• Horseradish

Some medicines may interfere with the test. These include vitamin C, aspirin, and NSAIDs such as ibuprofen and naproxen. Ask your provider if you need to stop taking these before the test. Never stop or change your medicine without first talking to your provider.

The at-home test involves a normal bowel movement. There is no discomfort.

You may have some discomfort if the stool is collected during a rectal exam.

This test detects blood in the digestive tract. It may be done if:

• You are being screened or tested for colon cancer.
• You have abdominal pain, changes in bowel movements, or weight loss.
• You have anemia (low blood count).
• You say you have blood in the stool or black, tarry stools.

A negative test result means that there is no blood in the stool. Occasionally, this test will miss very small amounts of blood in the stool that is below the level of detection of this test (false negative).

Abnormal results may be due to problems that cause bleeding in the stomach or intestinal tract, including:

• Colon cancer or other gastrointestinal tumors
• Colon polyps
• Bleeding veins in the esophagus or stomach (esophageal varices and portal hypertensive gastropathy)
• Inflammation of the esophagus (esophagitis)
• Inflammation of the stomach (gastritis)
• Hemorrhoids
• Crohn disease or ulcerative colitis
• Peptic ulcer

Other causes of a positive test may include:

• Nosebleed
• Coughing up blood and then swallowing it

If the stool guaiac results come back positive for blood in the stool, your provider will likely recommend other tests, often including a colonoscopy.

The stool guaiac test does not diagnose cancer. Screening tests such as colonoscopy can help detect cancer. The stool guaiac test and other screenings can catch colon cancer early, when it is easier to treat.

There can be false-positive and false-negative results.

Errors are reduced when you follow instructions during collection and avoid certain foods and medicines.

Colon cancer - guaiac test; Colorectal cancer - guaiac test; gFOBT; Guaiac smear test; Fecal occult blood test - guaiac smear; Stool occult blood test - guaiac smear

National Cancer Institute website. Colorectal cancer prevention (PDQ) - health professional version. www.cancer.gov/types/colorectal/hp/colorectal-prevention-pdq. Updated March 28, 2024. Accessed April 22, 2024.

National Comprehensive Cancer Network website. NCCN clinical practice guidelines in oncology (NCCN guidelines): colorectal cancer screening. Version 1.2024 - February 27, 2027. www.nccn.org/professionals/physician_gls/pdf/colorectal_screening.pdf. Accessed April 22, 2024.

Patel SG, May FP, Anderson JC, et al. Updates on age to start and stop colorectal cancer screening: recommendations from the U.S. Multi-Society Task Force on colorectal cancer. Gastroenterology. 2022;162(1):285-299. PMID: 34794816 pubmed.ncbi.nlm.nih.gov/34794816/.

Savides TJ, Jensen DM. Gastrointestinal bleeding. In: Feldman M, Friedman LS, Brandt LJ, eds. Sleisenger and Fordtran's Gastrointestinal and Liver Disease. 11th ed. Philadelphia, PA: Elsevier; 2021:chap 20.

US Preventive Services Task Force website. Final recommendation statement. Colorectal cancer: screening. www.uspreventiveservicestaskforce.org/uspstf/recommendation/colorectal-cancer-screening. Published May 18, 2021. Accessed February 11, 2024.

Updated by: Michael M. Phillips, MD, Emeritus Professor of Medicine, The George Washington University School of Medicine, Washington, DC. Internal review and update on 02/10/2024 by David C. Dugdale, MD, Medical Director, Brenda Conaway, Editorial Director, and the A.D.A.M. Editorial team.