A blood sample is needed. This is most often taken through a vein. The procedure is called a venipuncture.

There is no special preparation.

When the needle is inserted to draw blood, some people feel slight pain. Others may feel only a prick or sting. Afterward, there may be some throbbing.

Total complement activity (CH50, CH100) looks at the overall activity of the complement system. In most cases, other tests that are more specific for the suspected disease are done first. C3 and C4 are the complement components measured most often.

A complement test may be used to monitor people with an autoimmune disorder. It is also used to see if treatment for their condition is working. For example, people with active lupus erythematosus may have lower-than-normal levels of the complement proteins C3 and C4.

Complement activity varies throughout the body. For example, in people with rheumatoid arthritis, complement activity in the blood may be normal or higher-than-normal, but much lower-than-normal in the joint fluid.

People with some bacterial blood infections and shock often have very low C3 and components of what's known as the alternative pathway. C3 is often also low in fungal infections and some parasitic infections, such as malaria.

The normal results for this test are:

• Total blood complement level: 41 to 90 hemolytic units
• C1 level: 14.9 to 22.1 mg/dL
• C3 levels: 88 to 201 mg/dL
• C4 levels: 15 to 45 mg/dL

Note: mg/dL = milligrams per deciliter.

Note: Normal value ranges may vary slightly among different laboratories. Talk to your health care provider about the meaning of your specific test results.

The examples above show the common measurements for results for these tests. Some laboratories use different measurements or may test different specimens.

Increased complement activity may be seen in:

• Cancer
• Certain infections
• Ulcerative colitis

Decreased complement activity may be seen in:

• Cirrhosis
• Glomerulonephritis
• Hereditary angioedema
• Hepatitis
• Kidney transplant rejection
• Lupus nephritis
• Malnutrition
• Systemic lupus erythematosus
• Rare inherited complement deficiencies

Risks associated with having blood drawn are slight, but may include:

• Excessive bleeding
• Fainting or feeling lightheaded
• Hematoma (blood accumulating under the skin)
• Infection (a slight risk any time the skin is broken)

The "complement cascade" is a series of reactions that take place in the blood. The cascade activates the complement proteins. The result is an attack unit that creates holes in the membrane of bacteria, killing them.

Complement assay; Complement proteins

Bean KV, Massey D, Gupta G. Mediators of inflammation: complement. In: McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 24th ed. Philadelphia, PA: Elsevier; 2022:chap 47.

Ram S. Complement and deficiencies. In: Bennett JE, Dolin R, Blaser MJ, eds. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. 9th ed. Philadelphia, PA: Elsevier; 2020:chap 9.

Sullivan KE, Grumach AS. The complement system. In: Burks AW, Holgate ST, O'Hehir RE, Broide DH, et al. eds. Middleton's Allergy: Principles and Practice. 9th ed. Philadelphia, PA: Elsevier; 2020:chap 8.

Updated by: Neil J. Gonter, MD, Assistant Professor of Medicine, Columbia University, NY and private practice specializing in Rheumatology at Rheumatology Associates of North Jersey, Teaneck, NJ. Review provided by VeriMed Healthcare Network. Also reviewed by David C. Dugdale, MD, Medical Director, Brenda Conaway, Editorial Director, and the A.D.A.M. Editorial team.